Use molecular technique to detect toxoplasmosis in placenta and aborted fetus in women in Al-Diwaniyah province

The current study included the detection of Toxoplasma gondii from aborted women Maternity and Childhood Teaching Hospital. It drew attention to human infection rate in order detect differentiation them with SAG3 gene. samples were collected during period October, 2021 until end March 2022 AL-Qadisyah province. Total 100 (placenta fetus) send laboratory veterinary medicine AL- Qadisiyah University toxoplasmosis by impression method all are positive. present a molecular surface antigen 3 (SAG3) using Nested Polymerase Chain Reaction (nPCR), results nPCR for gene showed that 18/24 (75 %) positive woman. NCBI-BLAST Homology Sequence identity (%) between local T. isolated cases have been deposited NCBI under following accession numbers (ON240954, ON240955, ON240956, ON240957, ON240958, ON240959, ON240960, ON240961, ON240962, ON240963) other global strains.